Recombinant DNA Principles and Methodologies

        This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.

        Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

        Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as

·  plasmid biology
·  nucleic acid biochemistry
·  restriction enzymes
·  cloning strategies
·  gel electrophoresis
·  southern and northern blotting
·  preparation of probes
·  phage lambda biology
·  cosmids and genome analysis
·  cloned gene expression
·  polymerase chain reaction
·  conventional and automated DNA sequencing
·  site-directed mutagenesis
·  and more!

        Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.