RT-PCR Protocols

For students, researchers, laboratory managers, and technologists from different scientific backgrounds, King (School of Science and Technology, U. of New England, Australia) compiles 20 protocols that detail reverse-transcription polymerase chain reaction (RT-PCR) techniques like competitive and nested RT-PCR, RT-PCR from single cells, and for cloning. This edition has been updated to include newer technologies like multiplex and real-time quantitative RT-PCR, RT-LATE-PCR, and recent advances in creating the optimum RT-PCR reaction, like RNA extraction, primer design, and reverse transcription. Materials and reagents, reproducible protocols, and troubleshooting and pitfalls advice are given by the group of scientists, who work in physiology, biophysics, microbiology, and other fields around the world. Annotation 穢2010 Book News, Inc., Portland, OR (booknews.com)