Light Sheet Fluorescence Microscopy

Light Sheet Fluorescence Microscopy

An indispensable guide to a novel, revolutionary fluorescence microscopy technique!

Light sheet fluorescence microscopy has revolutionized microscopy, since it allows scientists to perform experiments in an entirely different manner and to record data that had not been accessible before. With contributions from noted experts in the fields of physics, biology, and computer science, Light Sheet Fluorescence Microscopy is a unique guide that offers a practical approach to the subject, including information on the basics of light sheet fluorescence microscopy, instrumentation, applications, sample preparation, and data analysis.

Comprehensive in scope, the book is filled with the cutting-edge methods as well as valuable insider tips. Grounded in real-world applications, the book includes chapters from major manufacturers that explores their recent systems and developments. In addition, the book hightlights a discussion of a ＂do-it-yourself＂ light sheet microscope, making the technique affordable for every laboratory.

This important textbook:

• Serves as an easy-to-understand introduction to light sheet-based fluorescence
• Includes numerous tips and tricks for advanced practitioners
• Provides in-depth information on hardware and software solutions for a straightforward implementation of light sheet fluorescence microscopy in the lab
• Includes chapters from the major manufacturers including Zeiss, Leica, Lavision Biotech, Phase View, and Asi

Aimed at cell biologists, biophysicists, developmental biologists, and neuro-biologists, Light Sheet Fluorescence Microscopy offers a comprehensive overview of the most recent applications of this microscopy technique.

Emmanuel Reynaud has been Stokes Lecturer in Biology at University College Dublin, Ireland, since 2009. He received his Master’s degree in molecular biology from the Victor Segalen University of Bordeaux, and his doctorate in biology from the University Paris XI-Orsay, France. In 2002, he received an EMBO Long Term Fellowship and moved to the European Molecular Biology Laboratory in Heidelberg, Germany, where he developed new methods in cell biology including laser nanosurgery approaches to study cytoskeleton dynamics as well as Golgi biogenesis. He was also involved in the development of the Light Sheet based Fluorescence Microscopy as a member of the Light Microscopy Group headed by Ernst H.K. Stelzer at EMBL. He has coordinated the Imaging Platform for the Tara Oceans Expedition and developed commercial applications with Carl Zeiss Microimaging, AURA Optik and ANDOR Ltd.

Pavel Tomancak is Senior Permanent Research Group Leader at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany. After studying Molecular Biology and Genetics at the Masaryk University in Brno, Czech Republic, he did his PhD at the European Molecular Biology Laboratory (EMBL) in the field of Drosophila developmental genetics. During his post-doctoral time at the University of California in Berkeley at the laboratory of Gerald M. Rubin, he established image-based genome-scale resources for patterns of gene expression in Drosophila embryos. His laboratory in Dresden continues to study patterns of gene expression during development by combining molecular, imaging and image analysis techniques. The group has lead a significant technological development aiming towards more complete quantitative description of gene expression patterns using light sheet microscopy.