Intracellular Thermometry with Fluorescent Molecular Thermometers

Intracellular Thermometry with Fluorescent Molecular Thermometers

Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale

Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology.

Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications.

Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find:

• Step by step instructions for constructing an intracellular thermometry experiment and validating results
• Comprehensive discussion of existing applications
• A vision for the future development of thermal biology as an independent discipline

Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

Seiichi Uchiyama has been an Assistant Professor at the University of Tokyo since 2005. After receiving his academic degrees in Pharmacy from the University of Tokyo with Prof. Kazuhiro Imai he spent three years as a postdoctoral researcher at Nara Women’s University with Prof. Kaoru Iwai and at Queen’s University of Belfast with Prof. A. Prasanna de Silva. After returning to the University of Tokyo in 2005, he started his career as an experimental researcher and continues to work as an independent leader of scientific projects funded by the Japanese government. His scientific interests include analytical and photophysical chemistry and the development of fluorescent sensors based on novel functional mechanisms.