Species of the beetle genus Diabrotica (Coleoptera: Chrysomelidae) are native to North and South America, with their greatest diversity occurring in neotropical areas. Little is known about the biology and ecology of many of the 400 described species, with current knowledge primarily limited to the small number of species that are pests in agricultural systems. This Special Issue, entitled "Corn Rootworm: Biology, Ecology, Behavior and Integrated Management", collates original research and comprehensive reviews that summarize the current knowledge in key areas of Diabrotica biology, ecology, behavior, and management.