This book presents the process of immobilizing lipase in a mesoporous material such as MCM-48, using ionic solids as the material’s structure drivers. It also presents the main applications of lipases, as well as the difficulties presented by using these enzymes in their free form. An alternative to overcome the limitations is the process of immobilization in supports that maintain the catalytic activities of the enzyme. Mesoporous materials of the MCM-48 type are an option as enzyme immobilization supports because they are inert and have little or no interference with the enzyme’s behaviour. The results obtained in this study showed that immobilization by the technique used was effective, allowing the enzyme to be trapped inside the crystalline network of the material, and the MCM-48 support proved to be efficient and promising, mainly due to its cost-effectiveness, making it potential for industrial applications. This book is suitable for students and researchers in the fields of chemistry and biotechnology.