The manual is written according to the PCI Syllabus of practical biochemistry for the Ist year pharmacy students. This book mainly guides students to get knowledge about basis of biochemistry. Students will find it a clear and concise overview of Biochemistry laboratory work. Most of the informations in this book has represented in a very simple manner. The aim of this book is to make the subject easy and understandable to the students. It aims at giving a good exposure of various aspects of biochemistry including the role of biochemistry in Pharmacy.These practical books mainly aim in guiding the under graduate and graduate students and overcoming the obstacles faced by them in practical aspects of Biochemistry. The major objective of this practical book is to present the information in alucid condensed manner to fulfill the requirements of students. This practical book tries to cover the syllabus of Biochemistry from most of the university from India.