Fruits and Vegetables are the food we eat every day. It is usually listed as an entire fruit or vegetable, juice, beverage or salad, etc., on diet charts worldwide. Fruit and Vegetables are a great source of key nutrients such as vitamins, minerals, fibers, and antioxidants that help prevent diabetes, cancer, and heart disease. Vegetables and fruits develop differently depending on various times of the year that are ideal for their growth conditions. Fresh fruits and vegetables carry an abundance of bacteria on their surfaces, not all of which cause disease. Contaminations come from various sources, including soil, water, insects, and air, from birds, animals, and machinery used in agriculture advertising them. The demand for food that is microbiologically safe has been highlighted in recent years due to rising consumer awareness. In this research primary goal was to isolate microorganisms from fresh fruit and vegetable samples and to specify the enumeration of bacteria in fresh fruit available in the Ayodhya market, Uttar Pradesh, India.