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This book provides a comprehensive overview of the application of microfluidic technology in the analysis and manipulation of mammalian cells. It introduces the fundamental principles of microfluidics and explores their use in measuring and controlling the biochemical and biophysical properties of cells for disease diagnosis and therapeutic development.

Microfluidic Technologies for Cell Analysis and Beyond centers on the comprehensive analysis of cells using microfluidic systems. It discusses microfluidics and their applications in the biochemical and biophysical analysis of both single cells and cell populations. Accessible to readers without a specialized background, Part I establishes foundations through cell analysis history and microfluidic fundamentals. Part II explores biochemical analysis advancements from single-cell proteomics to secretome profiling. Part III examines cellular biophysical properties including stiffness and migration, while Part IV covers manipulation techniques for cell movement and intracellular delivery. These chapters demonstrate how microfluidic technologies advance biology and translational medicine through adaptability and precision.

This comprehensive guide serves as a key reference for researchers, students, and professionals at the intersection of biology, engineering, and medicine.

Dr. Zongjie (Daniel) Wang is a Group Leader at the Chan Zuckerberg Biohub Network and an affiliated member of Northwestern University. His research focuses on the development and application of microfluidic systems for next-generation precision medicine, including high-throughput platforms for cell analysis and their integration into cancer immunotherapy and biomolecular screening. Dr. Wang has co-authored over 50 peer-reviewed articles, which have collectively received more than 5,000 citations. His pioneering work and perspectives have been published in leading journal, and he is the recipient of numerous honors, including the UBC Young Alumni Achievement Award, the ISAC President’s Award for Excellence, the Dimitris N. Chorafas Foundation Prize, and the Extraordinary Potential Award from the China Scholarship Council. Dr. Wang is also a co-founder of CTRL Therapeutics, a startup advancing microfluidic cell-sorting technologies for cancer immunotherapy.